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Effects of Experemental Cerebral Ishemia on Metabolic Characteristics of Parietal Cortex Neurons
Lizaveta Igorevna Bon,
Nataliya Yevgenyevna Maksimovich,
Sergey Michailovich Zimatkin
Issue:
Volume 2, Issue 1, March 2018
Pages:
1-5
Received:
21 February 2018
Accepted:
9 March 2018
Published:
3 April 2018
Abstract: The aim of the paper was to estimate the effect of experimental cerebral ischemia on metabolic characteristics of parietal cortex neurons. The experiments were carried out on 12 laboratory male rats. Cerebral ischemia was modeled with bilateral joint carotid arteries with durations of 60 minutes under intravenous thiopental anesthesia (40-50 mg / kg). The rats were decapitated and the samples of the brain parietal cortex were prepared for histological and histochemical examination in combination with morphometry to examine the 5th layer inner pyramidal neurons. It was increased the number of pathological forms and shrinkage of the 5th layer inner pyramidal neurons after cerebral ischemia in rats. The histochemical examination was revealed the inhibition of NADH-, glucose-6-phosphate dehydrogenase and succinate dehydrogenases as well as activation of lactate dehydrogenase and acid phosphatase. Cerebral ischemia induces deep histological and histochemical changes in the parietal cortex neurons in rats, including shrinkage of parietal cortex neurons and disturbances of their energy metabolism.
Abstract: The aim of the paper was to estimate the effect of experimental cerebral ischemia on metabolic characteristics of parietal cortex neurons. The experiments were carried out on 12 laboratory male rats. Cerebral ischemia was modeled with bilateral joint carotid arteries with durations of 60 minutes under intravenous thiopental anesthesia (40-50 mg / k...
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Influence of Cerebral Ischemia of Various Duration on Mitochondrial Respiration
Nataliya Yaugenievna Maksimovich,
Tatsiana Sergeevna Milosh,
Iosif Karlovich Dremza
Issue:
Volume 2, Issue 1, March 2018
Pages:
6-9
Received:
21 February 2018
Accepted:
11 March 2018
Published:
8 April 2018
Abstract: Estimation of the energy exchange parameters during partial brain ischemia offers an opportunity for finding new ways of correcting the above pathology. The aim of the study was to investigate the changes in mitochondrial respiration in the homogenates of rats with brain cerebral ischemia of various duration. The experiment was carried out on 19 white female rats in whom the right common carotid artery was ligated for 1 hour or 3 days with subsequent decapitation and brain removal. The rate of mitochondrial respiration in mitochondrial suspension of brain homogenates was assessed by means of the polarographic method using succinate or a mixture of L-malate/L-glutamate and adenosine diphosphate as respiratory substrates. Ligation of the right common carotid artery of various duration resulted in reduced mitochondrial respiration rate which was more pronounced after 1 hour of partial brain ischemia as compared to 3 hours. Modeling of partial cerebral ischemia in rats by ligation of the right common carotid artery considerably inhibits respiratory activity of the brain mitochondria leading to the dissociation of oxidation and phosphorylation in the mitochondria and inducing impairment of neuronal bioenergetics in cerebral tissue due to the disturbance of the supply of oxygen by blood. Understanding of pathophysiological processes in mitochondria during partial brain ischemia will help to develop new methods of pathogenetic correction of changes in cell structures of the brain tissue. This approach offers new possibilities for the pathogenetic therapy.
Abstract: Estimation of the energy exchange parameters during partial brain ischemia offers an opportunity for finding new ways of correcting the above pathology. The aim of the study was to investigate the changes in mitochondrial respiration in the homogenates of rats with brain cerebral ischemia of various duration. The experiment was carried out on 19 wh...
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Effect of Carbon Depth in Diminution of Physico-Chemical Pollution Parameters in Cassava Processing Wastewater Treated by Aeration-Peroxide Oxidation Method
Olayinka Omotosho,
Gbolabo Ogunwande,
Jimmy Osunbitan
Issue:
Volume 2, Issue 1, March 2018
Pages:
10-13
Received:
28 February 2018
Accepted:
19 March 2018
Published:
10 April 2018
Abstract: This study investigated the effect (attenuation of physico-chemical pollution characteristics) of carbon depth in vertical flow adsorption-filter system of the peroxide oxidation-aeration treatment process for cassava wastewater treatment. Results revealed that although the combined peroxide oxidation, aeration and adsorption-filter treatment process was successful in the reducing pollution characteristics of wastewater from cassava processing, the adsorption-filter part of the treatment process with carbon depths 0, 5, 10 and 15 cm carbon depths were observed to have been selective in removal of physico-chemical pollution characteristics. Parameters such as colour, SO42-, NO3-, Cl-, Ca2+, Zn2+, TDS and TSS were observed to have exhibited increased decontamination efficiency as the depth of carbon increased in the treatment process while Fe2+ and CN ions exhibited delayed adsorption. Depth of carbon filter was however observed to have had no effect on Ni2+ and Phenol content removal.
Abstract: This study investigated the effect (attenuation of physico-chemical pollution characteristics) of carbon depth in vertical flow adsorption-filter system of the peroxide oxidation-aeration treatment process for cassava wastewater treatment. Results revealed that although the combined peroxide oxidation, aeration and adsorption-filter treatment proce...
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Comparing the Sensitivity of Microscopy to Diagnostic Strip in the Survey for Urinary Schistosomiasis
Nathaniel Yacham Musa,
Anthony John Dadah
Issue:
Volume 2, Issue 1, March 2018
Pages:
14-19
Received:
1 March 2018
Accepted:
21 March 2018
Published:
12 April 2018
Abstract: Schistosomiasis is one of the Neglected Tropical Diseases (NTDs) recording high morbidity and mortality rate for developing countries in Africa, South America, the Caribbean, the Middle East, and Asia. The sensitivity of microscopy to the use of diagnostic strip in the survey for urinary schistosomiasis among students attending some selected secondary schools was investigated in Chikun Local Government Area of Kaduna State, Nigeria. Four hundred and sixty two (462) urine samples were collected and examined for the eggs of Schistosoma haematobium using sedimentation and microscopy method. Microhaematuria and proteinuria were investigated using combi 11 test strips. This study revealed 11.5% prevalence rate for urinary schistosomiasis among the studied subjects based on direct egg detection method, 22.9% based on micro-haematuria detection and 44.8% based on proteinuria. The sensitivity of Urine dip strip when compared to direct egg detection methods, shows that there is high sensitivity on the use of urine dip strip.
Abstract: Schistosomiasis is one of the Neglected Tropical Diseases (NTDs) recording high morbidity and mortality rate for developing countries in Africa, South America, the Caribbean, the Middle East, and Asia. The sensitivity of microscopy to the use of diagnostic strip in the survey for urinary schistosomiasis among students attending some selected second...
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