Influence of Cerebral Ischemia of Various Duration on Mitochondrial Respiration

Nataliya Yaugenievna Maksimovich; Tatsiana Sergeevna Milosh; Iosif Karlovich Dremza

doi:doi:10.11648/j.be.20180201.12

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Influence of Cerebral Ischemia of Various Duration on Mitochondrial Respiration

Nataliya Yaugenievna Maksimovich, Tatsiana Sergeevna Milosh, Iosif Karlovich Dremza

Published in Bioprocess Engineering (Volume 2, Issue 1)

Received: 21 February 2018 Accepted: 11 March 2018 Published: 8 April 2018

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Abstract

Estimation of the energy exchange parameters during partial brain ischemia offers an opportunity for finding new ways of correcting the above pathology. The aim of the study was to investigate the changes in mitochondrial respiration in the homogenates of rats with brain cerebral ischemia of various duration. The experiment was carried out on 19 white female rats in whom the right common carotid artery was ligated for 1 hour or 3 days with subsequent decapitation and brain removal. The rate of mitochondrial respiration in mitochondrial suspension of brain homogenates was assessed by means of the polarographic method using succinate or a mixture of L-malate/L-glutamate and adenosine diphosphate as respiratory substrates. Ligation of the right common carotid artery of various duration resulted in reduced mitochondrial respiration rate which was more pronounced after 1 hour of partial brain ischemia as compared to 3 hours. Modeling of partial cerebral ischemia in rats by ligation of the right common carotid artery considerably inhibits respiratory activity of the brain mitochondria leading to the dissociation of oxidation and phosphorylation in the mitochondria and inducing impairment of neuronal bioenergetics in cerebral tissue due to the disturbance of the supply of oxygen by blood. Understanding of pathophysiological processes in mitochondria during partial brain ischemia will help to develop new methods of pathogenetic correction of changes in cell structures of the brain tissue. This approach offers new possibilities for the pathogenetic therapy.

Published in	Bioprocess Engineering (Volume 2, Issue 1)
DOI	10.11648/j.be.20180201.12
Page(s)	6-9
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2018. Published by Science Publishing Group

Keywords

Rats, Brain, Partial Ischemia, Mitochondrial Respiration

References

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[2]	Bova AA, Silinovich MV (2015) Ischemic stroke: the strategy of patient management (compliance of recommendations and their real clinical application) Military medicine. 4 (37): 30–35.
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[4]	Gusev EI, Martynov MYu, Kamchatnov PR (2013) Ischemic stroke. Current state of the problem. Doktor. ru. Moscow, 5 (83): 2–7.
[5]	Hossman KA (1998) Experimental models for the investigation of brain ischemia Cardiovascular Resarch. 39: 106–120.
[6]	Lai JCK, Clark JB (1974) Preparation of synaptic and nonsynaptic mitochondria from mammalian brain Methods in Enzymology. 60: 51–64.
[7]	Maksimovich NE (2003) The concept of the nitroxidergic system of the brain. The role of neuronal sources Journal of the Grodno State Medical University. 4: 7–11.
[8]	Maksimovich NE (2004) Peculiarities of formation of the level of azoate oxide in rat blood plasma in ischemic and reperfusion injuries of the brain Regional circulation and microcirculation. 3 (11): 55–60.
[9]	Maksimovich NE (2010) Modern ideas about the mechanisms of development of ischemic brain damage and ways of correction of this pathology Actual theoretical and applied aspects of pathophysiology: materials. Republican scientific-practical conference with international participation May 14, 2010–Grodno: 149–157.
[10]	Rukan TA, Maksimovich NE, Zimatkin SM (2012) Morphofunctional changes in the neurons of the frontal cortex of rat brain under conditions of its ischemia-reperfusion Journal of the Grodno State Medical University. 4: 35–38.
[11]	Solodkov AP, Chirkin AA (2010) Modern problems of biochemistry: textbook Vitebsk: "VSU named after P. M. Masherov ": 384.
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Nataliya Yaugenievna Maksimovich, Tatsiana Sergeevna Milosh, Iosif Karlovich Dremza. (2018). Influence of Cerebral Ischemia of Various Duration on Mitochondrial Respiration. Bioprocess Engineering, 2(1), 6-9. https://doi.org/10.11648/j.be.20180201.12

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ACS Style

Nataliya Yaugenievna Maksimovich; Tatsiana Sergeevna Milosh; Iosif Karlovich Dremza. Influence of Cerebral Ischemia of Various Duration on Mitochondrial Respiration. Bioprocess Eng. 2018, 2(1), 6-9. doi: 10.11648/j.be.20180201.12

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AMA Style

Nataliya Yaugenievna Maksimovich, Tatsiana Sergeevna Milosh, Iosif Karlovich Dremza. Influence of Cerebral Ischemia of Various Duration on Mitochondrial Respiration. Bioprocess Eng. 2018;2(1):6-9. doi: 10.11648/j.be.20180201.12

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@article{10.11648/j.be.20180201.12,
  author = {Nataliya Yaugenievna Maksimovich and Tatsiana Sergeevna Milosh and Iosif Karlovich Dremza},
  title = {Influence of Cerebral Ischemia of Various Duration on Mitochondrial Respiration},
  journal = {Bioprocess Engineering},
  volume = {2},
  number = {1},
  pages = {6-9},
  doi = {10.11648/j.be.20180201.12},
  url = {https://doi.org/10.11648/j.be.20180201.12},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.be.20180201.12},
  abstract = {Estimation of the energy exchange parameters during partial brain ischemia offers an opportunity for finding new ways of correcting the above pathology. The aim of the study was to investigate the changes in mitochondrial respiration in the homogenates of rats with brain cerebral ischemia of various duration. The experiment was carried out on 19 white female rats in whom the right common carotid artery was ligated for 1 hour or 3 days with subsequent decapitation and brain removal. The rate of mitochondrial respiration in mitochondrial suspension of brain homogenates was assessed by means of the polarographic method using succinate or a mixture of L-malate/L-glutamate and adenosine diphosphate as respiratory substrates. Ligation of the right common carotid artery of various duration resulted in reduced mitochondrial respiration rate which was more pronounced after 1 hour of partial brain ischemia as compared to 3 hours. Modeling of partial cerebral ischemia in rats by ligation of the right common carotid artery considerably inhibits respiratory activity of the brain mitochondria leading to the dissociation of oxidation and phosphorylation in the mitochondria and inducing impairment of neuronal bioenergetics in cerebral tissue due to the disturbance of the supply of oxygen by blood. Understanding of pathophysiological processes in mitochondria during partial brain ischemia will help to develop new methods of pathogenetic correction of changes in cell structures of the brain tissue. This approach offers new possibilities for the pathogenetic therapy.},
 year = {2018}
}

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TY  - JOUR
T1  - Influence of Cerebral Ischemia of Various Duration on Mitochondrial Respiration
AU  - Nataliya Yaugenievna Maksimovich
AU  - Tatsiana Sergeevna Milosh
AU  - Iosif Karlovich Dremza
Y1  - 2018/04/08
PY  - 2018
N1  - https://doi.org/10.11648/j.be.20180201.12
DO  - 10.11648/j.be.20180201.12
T2  - Bioprocess Engineering
JF  - Bioprocess Engineering
JO  - Bioprocess Engineering
SP  - 6
EP  - 9
PB  - Science Publishing Group
SN  - 2578-8701
UR  - https://doi.org/10.11648/j.be.20180201.12
AB  - Estimation of the energy exchange parameters during partial brain ischemia offers an opportunity for finding new ways of correcting the above pathology. The aim of the study was to investigate the changes in mitochondrial respiration in the homogenates of rats with brain cerebral ischemia of various duration. The experiment was carried out on 19 white female rats in whom the right common carotid artery was ligated for 1 hour or 3 days with subsequent decapitation and brain removal. The rate of mitochondrial respiration in mitochondrial suspension of brain homogenates was assessed by means of the polarographic method using succinate or a mixture of L-malate/L-glutamate and adenosine diphosphate as respiratory substrates. Ligation of the right common carotid artery of various duration resulted in reduced mitochondrial respiration rate which was more pronounced after 1 hour of partial brain ischemia as compared to 3 hours. Modeling of partial cerebral ischemia in rats by ligation of the right common carotid artery considerably inhibits respiratory activity of the brain mitochondria leading to the dissociation of oxidation and phosphorylation in the mitochondria and inducing impairment of neuronal bioenergetics in cerebral tissue due to the disturbance of the supply of oxygen by blood. Understanding of pathophysiological processes in mitochondria during partial brain ischemia will help to develop new methods of pathogenetic correction of changes in cell structures of the brain tissue. This approach offers new possibilities for the pathogenetic therapy.
VL  - 2
IS  - 1
ER  -

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Author Information

Nataliya Yaugenievna Maksimovich

Department of Pathophysiology of the Name of D. A. Maslakov, Grodno State Medical University, Grodno, Belarus
Tatsiana Sergeevna Milosh

Department of Pathophysiology of the Name of D. A. Maslakov, Grodno State Medical University, Grodno, Belarus
Iosif Karlovich Dremza

Department of Pathophysiology of the Name of D. A. Maslakov, Grodno State Medical University, Grodno, Belarus

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APA Style

Nataliya Yaugenievna Maksimovich, Tatsiana Sergeevna Milosh, Iosif Karlovich Dremza. (2018). Influence of Cerebral Ischemia of Various Duration on Mitochondrial Respiration. Bioprocess Engineering, 2(1), 6-9. https://doi.org/10.11648/j.be.20180201.12

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ACS Style

Nataliya Yaugenievna Maksimovich; Tatsiana Sergeevna Milosh; Iosif Karlovich Dremza. Influence of Cerebral Ischemia of Various Duration on Mitochondrial Respiration. Bioprocess Eng. 2018, 2(1), 6-9. doi: 10.11648/j.be.20180201.12

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AMA Style

Nataliya Yaugenievna Maksimovich, Tatsiana Sergeevna Milosh, Iosif Karlovich Dremza. Influence of Cerebral Ischemia of Various Duration on Mitochondrial Respiration. Bioprocess Eng. 2018;2(1):6-9. doi: 10.11648/j.be.20180201.12

Copy | Download

@article{10.11648/j.be.20180201.12,
  author = {Nataliya Yaugenievna Maksimovich and Tatsiana Sergeevna Milosh and Iosif Karlovich Dremza},
  title = {Influence of Cerebral Ischemia of Various Duration on Mitochondrial Respiration},
  journal = {Bioprocess Engineering},
  volume = {2},
  number = {1},
  pages = {6-9},
  doi = {10.11648/j.be.20180201.12},
  url = {https://doi.org/10.11648/j.be.20180201.12},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.be.20180201.12},
  abstract = {Estimation of the energy exchange parameters during partial brain ischemia offers an opportunity for finding new ways of correcting the above pathology. The aim of the study was to investigate the changes in mitochondrial respiration in the homogenates of rats with brain cerebral ischemia of various duration. The experiment was carried out on 19 white female rats in whom the right common carotid artery was ligated for 1 hour or 3 days with subsequent decapitation and brain removal. The rate of mitochondrial respiration in mitochondrial suspension of brain homogenates was assessed by means of the polarographic method using succinate or a mixture of L-malate/L-glutamate and adenosine diphosphate as respiratory substrates. Ligation of the right common carotid artery of various duration resulted in reduced mitochondrial respiration rate which was more pronounced after 1 hour of partial brain ischemia as compared to 3 hours. Modeling of partial cerebral ischemia in rats by ligation of the right common carotid artery considerably inhibits respiratory activity of the brain mitochondria leading to the dissociation of oxidation and phosphorylation in the mitochondria and inducing impairment of neuronal bioenergetics in cerebral tissue due to the disturbance of the supply of oxygen by blood. Understanding of pathophysiological processes in mitochondria during partial brain ischemia will help to develop new methods of pathogenetic correction of changes in cell structures of the brain tissue. This approach offers new possibilities for the pathogenetic therapy.},
 year = {2018}
}

Copy | Download

TY  - JOUR
T1  - Influence of Cerebral Ischemia of Various Duration on Mitochondrial Respiration
AU  - Nataliya Yaugenievna Maksimovich
AU  - Tatsiana Sergeevna Milosh
AU  - Iosif Karlovich Dremza
Y1  - 2018/04/08
PY  - 2018
N1  - https://doi.org/10.11648/j.be.20180201.12
DO  - 10.11648/j.be.20180201.12
T2  - Bioprocess Engineering
JF  - Bioprocess Engineering
JO  - Bioprocess Engineering
SP  - 6
EP  - 9
PB  - Science Publishing Group
SN  - 2578-8701
UR  - https://doi.org/10.11648/j.be.20180201.12
AB  - Estimation of the energy exchange parameters during partial brain ischemia offers an opportunity for finding new ways of correcting the above pathology. The aim of the study was to investigate the changes in mitochondrial respiration in the homogenates of rats with brain cerebral ischemia of various duration. The experiment was carried out on 19 white female rats in whom the right common carotid artery was ligated for 1 hour or 3 days with subsequent decapitation and brain removal. The rate of mitochondrial respiration in mitochondrial suspension of brain homogenates was assessed by means of the polarographic method using succinate or a mixture of L-malate/L-glutamate and adenosine diphosphate as respiratory substrates. Ligation of the right common carotid artery of various duration resulted in reduced mitochondrial respiration rate which was more pronounced after 1 hour of partial brain ischemia as compared to 3 hours. Modeling of partial cerebral ischemia in rats by ligation of the right common carotid artery considerably inhibits respiratory activity of the brain mitochondria leading to the dissociation of oxidation and phosphorylation in the mitochondria and inducing impairment of neuronal bioenergetics in cerebral tissue due to the disturbance of the supply of oxygen by blood. Understanding of pathophysiological processes in mitochondria during partial brain ischemia will help to develop new methods of pathogenetic correction of changes in cell structures of the brain tissue. This approach offers new possibilities for the pathogenetic therapy.
VL  - 2
IS  - 1
ER  -

Copy | Download