Statistical Approach for the Production of Protease and Cellulase from <i>Bacillus cereus</i> KA3

Mani Kalaiyarasi; Ponnuswamy Vijayaraghavan; Subhanandharaj Russalamma Flanet Raj; Samuel Gnana Prakash Vincent

doi:doi:10.11648/j.be.20170104.11

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Statistical Approach for the Production of Protease and Cellulase from Bacillus cereus KA3

Mani Kalaiyarasi, Ponnuswamy Vijayaraghavan, Subhanandharaj Russalamma Flanet Raj, Samuel Gnana Prakash Vincent

Published in Bioprocess Engineering (Volume 1, Issue 4)

Received: 6 May 2017 Accepted: 12 June 2017 Published: 31 July 2017

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Abstract

A cheap agro-industrial waste was used as the substrate for the production of protease and cellulase from Bacillus cereus KA3. The process parameters were optimized by a two level full factorial design and response surface methodology. Two level full factorial designs revealed that the factors namely, pH, peptone and NaH₂PO₄ were significantly influenced on the production of protease and cellulase. These three significant factors were selected for central composite design and response surface methodology. The maximum protease and cellulase production was 3127 U/g, and 482 U/g, respectively, after statistical approach, which showed over fourfold increase in enzyme production than unoptimized medium.

Published in	Bioprocess Engineering (Volume 1, Issue 4)
DOI	10.11648/j.be.20170104.11
Page(s)	93-103
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2017. Published by Science Publishing Group

Keywords

Solid State Fermentation, Agro-residues, Response Surface Methodology, Optimization

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APA Style

Mani Kalaiyarasi, Ponnuswamy Vijayaraghavan, Subhanandharaj Russalamma Flanet Raj, Samuel Gnana Prakash Vincent. (2017). Statistical Approach for the Production of Protease and Cellulase from Bacillus cereus KA3. Bioprocess Engineering, 1(4), 93-103. https://doi.org/10.11648/j.be.20170104.11

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ACS Style

Mani Kalaiyarasi; Ponnuswamy Vijayaraghavan; Subhanandharaj Russalamma Flanet Raj; Samuel Gnana Prakash Vincent. Statistical Approach for the Production of Protease and Cellulase from Bacillus cereus KA3. Bioprocess Eng. 2017, 1(4), 93-103. doi: 10.11648/j.be.20170104.11

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AMA Style

Mani Kalaiyarasi, Ponnuswamy Vijayaraghavan, Subhanandharaj Russalamma Flanet Raj, Samuel Gnana Prakash Vincent. Statistical Approach for the Production of Protease and Cellulase from Bacillus cereus KA3. Bioprocess Eng. 2017;1(4):93-103. doi: 10.11648/j.be.20170104.11

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@article{10.11648/j.be.20170104.11,
  author = {Mani Kalaiyarasi and Ponnuswamy Vijayaraghavan and Subhanandharaj Russalamma Flanet Raj and Samuel Gnana Prakash Vincent},
  title = {Statistical Approach for the Production of Protease and Cellulase from Bacillus cereus KA3},
  journal = {Bioprocess Engineering},
  volume = {1},
  number = {4},
  pages = {93-103},
  doi = {10.11648/j.be.20170104.11},
  url = {https://doi.org/10.11648/j.be.20170104.11},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.be.20170104.11},
  abstract = {A cheap agro-industrial waste was used as the substrate for the production of protease and cellulase from Bacillus cereus KA3. The process parameters were optimized by a two level full factorial design and response surface methodology. Two level full factorial designs revealed that the factors namely, pH, peptone and NaH2PO4 were significantly influenced on the production of protease and cellulase. These three significant factors were selected for central composite design and response surface methodology. The maximum protease and cellulase production was 3127 U/g, and 482 U/g, respectively, after statistical approach, which showed over fourfold increase in enzyme production than unoptimized medium.},
 year = {2017}
}

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TY  - JOUR
T1  - Statistical Approach for the Production of Protease and Cellulase from Bacillus cereus KA3
AU  - Mani Kalaiyarasi
AU  - Ponnuswamy Vijayaraghavan
AU  - Subhanandharaj Russalamma Flanet Raj
AU  - Samuel Gnana Prakash Vincent
Y1  - 2017/07/31
PY  - 2017
N1  - https://doi.org/10.11648/j.be.20170104.11
DO  - 10.11648/j.be.20170104.11
T2  - Bioprocess Engineering
JF  - Bioprocess Engineering
JO  - Bioprocess Engineering
SP  - 93
EP  - 103
PB  - Science Publishing Group
SN  - 2578-8701
UR  - https://doi.org/10.11648/j.be.20170104.11
AB  - A cheap agro-industrial waste was used as the substrate for the production of protease and cellulase from Bacillus cereus KA3. The process parameters were optimized by a two level full factorial design and response surface methodology. Two level full factorial designs revealed that the factors namely, pH, peptone and NaH2PO4 were significantly influenced on the production of protease and cellulase. These three significant factors were selected for central composite design and response surface methodology. The maximum protease and cellulase production was 3127 U/g, and 482 U/g, respectively, after statistical approach, which showed over fourfold increase in enzyme production than unoptimized medium.
VL  - 1
IS  - 4
ER  -

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Author Information

Mani Kalaiyarasi

International Centre for Nanobiotechnology, Centre for Marine Science and Technology, Manonmaniam Sundaranar University, Kanyakumari District, Tamilnadu, India
Ponnuswamy Vijayaraghavan

International Centre for Nanobiotechnology, Centre for Marine Science and Technology, Manonmaniam Sundaranar University, Kanyakumari District, Tamilnadu, India
Subhanandharaj Russalamma Flanet Raj

Department of Zoology, Nesamony Memorial Christian College, Kanyakumari District, Tamilnadu, India
Samuel Gnana Prakash Vincent

International Centre for Nanobiotechnology, Centre for Marine Science and Technology, Manonmaniam Sundaranar University, Kanyakumari District, Tamilnadu, India

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APA Style

Mani Kalaiyarasi, Ponnuswamy Vijayaraghavan, Subhanandharaj Russalamma Flanet Raj, Samuel Gnana Prakash Vincent. (2017). Statistical Approach for the Production of Protease and Cellulase from Bacillus cereus KA3. Bioprocess Engineering, 1(4), 93-103. https://doi.org/10.11648/j.be.20170104.11

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ACS Style

Mani Kalaiyarasi; Ponnuswamy Vijayaraghavan; Subhanandharaj Russalamma Flanet Raj; Samuel Gnana Prakash Vincent. Statistical Approach for the Production of Protease and Cellulase from Bacillus cereus KA3. Bioprocess Eng. 2017, 1(4), 93-103. doi: 10.11648/j.be.20170104.11

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AMA Style

Mani Kalaiyarasi, Ponnuswamy Vijayaraghavan, Subhanandharaj Russalamma Flanet Raj, Samuel Gnana Prakash Vincent. Statistical Approach for the Production of Protease and Cellulase from Bacillus cereus KA3. Bioprocess Eng. 2017;1(4):93-103. doi: 10.11648/j.be.20170104.11

Copy | Download

@article{10.11648/j.be.20170104.11,
  author = {Mani Kalaiyarasi and Ponnuswamy Vijayaraghavan and Subhanandharaj Russalamma Flanet Raj and Samuel Gnana Prakash Vincent},
  title = {Statistical Approach for the Production of Protease and Cellulase from Bacillus cereus KA3},
  journal = {Bioprocess Engineering},
  volume = {1},
  number = {4},
  pages = {93-103},
  doi = {10.11648/j.be.20170104.11},
  url = {https://doi.org/10.11648/j.be.20170104.11},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.be.20170104.11},
  abstract = {A cheap agro-industrial waste was used as the substrate for the production of protease and cellulase from Bacillus cereus KA3. The process parameters were optimized by a two level full factorial design and response surface methodology. Two level full factorial designs revealed that the factors namely, pH, peptone and NaH2PO4 were significantly influenced on the production of protease and cellulase. These three significant factors were selected for central composite design and response surface methodology. The maximum protease and cellulase production was 3127 U/g, and 482 U/g, respectively, after statistical approach, which showed over fourfold increase in enzyme production than unoptimized medium.},
 year = {2017}
}

Copy | Download

TY  - JOUR
T1  - Statistical Approach for the Production of Protease and Cellulase from Bacillus cereus KA3
AU  - Mani Kalaiyarasi
AU  - Ponnuswamy Vijayaraghavan
AU  - Subhanandharaj Russalamma Flanet Raj
AU  - Samuel Gnana Prakash Vincent
Y1  - 2017/07/31
PY  - 2017
N1  - https://doi.org/10.11648/j.be.20170104.11
DO  - 10.11648/j.be.20170104.11
T2  - Bioprocess Engineering
JF  - Bioprocess Engineering
JO  - Bioprocess Engineering
SP  - 93
EP  - 103
PB  - Science Publishing Group
SN  - 2578-8701
UR  - https://doi.org/10.11648/j.be.20170104.11
AB  - A cheap agro-industrial waste was used as the substrate for the production of protease and cellulase from Bacillus cereus KA3. The process parameters were optimized by a two level full factorial design and response surface methodology. Two level full factorial designs revealed that the factors namely, pH, peptone and NaH2PO4 were significantly influenced on the production of protease and cellulase. These three significant factors were selected for central composite design and response surface methodology. The maximum protease and cellulase production was 3127 U/g, and 482 U/g, respectively, after statistical approach, which showed over fourfold increase in enzyme production than unoptimized medium.
VL  - 1
IS  - 4
ER  -

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