<i>Xanthomonas albilineans</i> (Ashby) Dowson: Study of Its Genetic Variability in Sugarcane Samples in Cuba

Yosel Perez Perez; Jose Ramon Perez Milian; Maria La O Hechevarria; Yaquelin Puchades Izaguirre; Yenima Pellon Guzman

doi:doi:10.11648/j.be.20210501.15

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Xanthomonas albilineans (Ashby) Dowson: Study of Its Genetic Variability in Sugarcane Samples in Cuba

Yosel Perez Perez, Jose Ramon Perez Milian, Maria La O Hechevarria, Yaquelin Puchades Izaguirre, Yenima Pellon Guzman

Published in Bioprocess Engineering (Volume 5, Issue 1)

Received: 22 October 2020 Accepted: 7 November 2020 Published: 25 June 2021

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Abstract

Four isolates of the phytopathogenic bacterium Xanthomonas albilineans (Xa) from samples taken in commercial fields of different Cuban provinces and two from the cultivar L55-5, artificially infected in the Camagüey and Matanzas Experimental Stations, were used for the extraction of total nucleic acids according to the CTAB protocol. They were amplified by PCR using four primer combinations: A: P1-M1, B: P1-M2, C: P2-M1 and D: P2-M2. Direct sequencing of the PCR products was performed on all samples and the nucleotide sequences were used to generate partial consensus of 16-23S rRNA, which were analyzed with the BLASTn program. In all the isolates evaluated, the presence of a common band at the height of 620 bp was visualized and the AFLP profile showed that there is homology when comparing the sequences corresponding to the 16-23S rDNA of X. albilineans of the different isolates; the phylogenetic analysis reflected a grouping of the isolates of the species, regardless of its geographical origin, different from others of the genus Xanthomonas. The comparison of the Cuban isolates with eight strains published in GenBank, showed marked similarity with them, with 100% nucleotide identity and percentage of coverage with a genome fragment of the GPE PC73 strain from Guadalupe, KO905 from the United States and a strain from Brazil. The predominance of the generalized circulation in Cuba of a single serovar is presumed.

Published in	Bioprocess Engineering (Volume 5, Issue 1)
DOI	10.11648/j.be.20210501.15
Page(s)	23-27
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2021. Published by Science Publishing Group

Keywords

Xanthomonas albilineans, PCR, Sequence, Variability, Identity

References

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APA Style

Yosel Perez Perez, Jose Ramon Perez Milian, Maria La O Hechevarria, Yaquelin Puchades Izaguirre, Yenima Pellon Guzman. (2021). Xanthomonas albilineans (Ashby) Dowson: Study of Its Genetic Variability in Sugarcane Samples in Cuba. Bioprocess Engineering, 5(1), 23-27. https://doi.org/10.11648/j.be.20210501.15

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ACS Style

Yosel Perez Perez; Jose Ramon Perez Milian; Maria La O Hechevarria; Yaquelin Puchades Izaguirre; Yenima Pellon Guzman. Xanthomonas albilineans (Ashby) Dowson: Study of Its Genetic Variability in Sugarcane Samples in Cuba. Bioprocess Eng. 2021, 5(1), 23-27. doi: 10.11648/j.be.20210501.15

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AMA Style

Yosel Perez Perez, Jose Ramon Perez Milian, Maria La O Hechevarria, Yaquelin Puchades Izaguirre, Yenima Pellon Guzman. Xanthomonas albilineans (Ashby) Dowson: Study of Its Genetic Variability in Sugarcane Samples in Cuba. Bioprocess Eng. 2021;5(1):23-27. doi: 10.11648/j.be.20210501.15

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@article{10.11648/j.be.20210501.15,
  author = {Yosel Perez Perez and Jose Ramon Perez Milian and Maria La O Hechevarria and Yaquelin Puchades Izaguirre and Yenima Pellon Guzman},
  title = {Xanthomonas albilineans (Ashby) Dowson: Study of Its Genetic Variability in Sugarcane Samples in Cuba},
  journal = {Bioprocess Engineering},
  volume = {5},
  number = {1},
  pages = {23-27},
  doi = {10.11648/j.be.20210501.15},
  url = {https://doi.org/10.11648/j.be.20210501.15},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.be.20210501.15},
  abstract = {Four isolates of the phytopathogenic bacterium Xanthomonas albilineans (Xa) from samples taken in commercial fields of different Cuban provinces and two from the cultivar L55-5, artificially infected in the Camagüey and Matanzas Experimental Stations, were used for the extraction of total nucleic acids according to the CTAB protocol. They were amplified by PCR using four primer combinations: A: P1-M1, B: P1-M2, C: P2-M1 and D: P2-M2. Direct sequencing of the PCR products was performed on all samples and the nucleotide sequences were used to generate partial consensus of 16-23S rRNA, which were analyzed with the BLASTn program. In all the isolates evaluated, the presence of a common band at the height of 620 bp was visualized and the AFLP profile showed that there is homology when comparing the sequences corresponding to the 16-23S rDNA of X. albilineans of the different isolates; the phylogenetic analysis reflected a grouping of the isolates of the species, regardless of its geographical origin, different from others of the genus Xanthomonas. The comparison of the Cuban isolates with eight strains published in GenBank, showed marked similarity with them, with 100% nucleotide identity and percentage of coverage with a genome fragment of the GPE PC73 strain from Guadalupe, KO905 from the United States and a strain from Brazil. The predominance of the generalized circulation in Cuba of a single serovar is presumed.},
 year = {2021}
}

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TY  - JOUR
T1  - Xanthomonas albilineans (Ashby) Dowson: Study of Its Genetic Variability in Sugarcane Samples in Cuba
AU  - Yosel Perez Perez
AU  - Jose Ramon Perez Milian
AU  - Maria La O Hechevarria
AU  - Yaquelin Puchades Izaguirre
AU  - Yenima Pellon Guzman
Y1  - 2021/06/25
PY  - 2021
N1  - https://doi.org/10.11648/j.be.20210501.15
DO  - 10.11648/j.be.20210501.15
T2  - Bioprocess Engineering
JF  - Bioprocess Engineering
JO  - Bioprocess Engineering
SP  - 23
EP  - 27
PB  - Science Publishing Group
SN  - 2578-8701
UR  - https://doi.org/10.11648/j.be.20210501.15
AB  - Four isolates of the phytopathogenic bacterium Xanthomonas albilineans (Xa) from samples taken in commercial fields of different Cuban provinces and two from the cultivar L55-5, artificially infected in the Camagüey and Matanzas Experimental Stations, were used for the extraction of total nucleic acids according to the CTAB protocol. They were amplified by PCR using four primer combinations: A: P1-M1, B: P1-M2, C: P2-M1 and D: P2-M2. Direct sequencing of the PCR products was performed on all samples and the nucleotide sequences were used to generate partial consensus of 16-23S rRNA, which were analyzed with the BLASTn program. In all the isolates evaluated, the presence of a common band at the height of 620 bp was visualized and the AFLP profile showed that there is homology when comparing the sequences corresponding to the 16-23S rDNA of X. albilineans of the different isolates; the phylogenetic analysis reflected a grouping of the isolates of the species, regardless of its geographical origin, different from others of the genus Xanthomonas. The comparison of the Cuban isolates with eight strains published in GenBank, showed marked similarity with them, with 100% nucleotide identity and percentage of coverage with a genome fragment of the GPE PC73 strain from Guadalupe, KO905 from the United States and a strain from Brazil. The predominance of the generalized circulation in Cuba of a single serovar is presumed.
VL  - 5
IS  - 1
ER  -

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Author Information

Yosel Perez Perez

Plant Protection Department, Sugarcane Research Institute (INICA), La Habana, Cuba
Jose Ramon Perez Milian

Plant Protection Department, Sugarcane Research Institute (INICA), La Habana, Cuba
Maria La O Hechevarria

Plant Protection Department, Sugarcane Research Institute (INICA), La Habana, Cuba
Yaquelin Puchades Izaguirre

Plant Protection Department, Sugarcane Research Institute (INICA), La Habana, Cuba
Yenima Pellon Guzman

Plant Protection Department, Sugarcane Research Institute (INICA), La Habana, Cuba

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Yosel Perez Perez, Jose Ramon Perez Milian, Maria La O Hechevarria, Yaquelin Puchades Izaguirre, Yenima Pellon Guzman. (2021). Xanthomonas albilineans (Ashby) Dowson: Study of Its Genetic Variability in Sugarcane Samples in Cuba. Bioprocess Engineering, 5(1), 23-27. https://doi.org/10.11648/j.be.20210501.15

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ACS Style

Yosel Perez Perez; Jose Ramon Perez Milian; Maria La O Hechevarria; Yaquelin Puchades Izaguirre; Yenima Pellon Guzman. Xanthomonas albilineans (Ashby) Dowson: Study of Its Genetic Variability in Sugarcane Samples in Cuba. Bioprocess Eng. 2021, 5(1), 23-27. doi: 10.11648/j.be.20210501.15

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AMA Style

Yosel Perez Perez, Jose Ramon Perez Milian, Maria La O Hechevarria, Yaquelin Puchades Izaguirre, Yenima Pellon Guzman. Xanthomonas albilineans (Ashby) Dowson: Study of Its Genetic Variability in Sugarcane Samples in Cuba. Bioprocess Eng. 2021;5(1):23-27. doi: 10.11648/j.be.20210501.15

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@article{10.11648/j.be.20210501.15,
  author = {Yosel Perez Perez and Jose Ramon Perez Milian and Maria La O Hechevarria and Yaquelin Puchades Izaguirre and Yenima Pellon Guzman},
  title = {Xanthomonas albilineans (Ashby) Dowson: Study of Its Genetic Variability in Sugarcane Samples in Cuba},
  journal = {Bioprocess Engineering},
  volume = {5},
  number = {1},
  pages = {23-27},
  doi = {10.11648/j.be.20210501.15},
  url = {https://doi.org/10.11648/j.be.20210501.15},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.be.20210501.15},
  abstract = {Four isolates of the phytopathogenic bacterium Xanthomonas albilineans (Xa) from samples taken in commercial fields of different Cuban provinces and two from the cultivar L55-5, artificially infected in the Camagüey and Matanzas Experimental Stations, were used for the extraction of total nucleic acids according to the CTAB protocol. They were amplified by PCR using four primer combinations: A: P1-M1, B: P1-M2, C: P2-M1 and D: P2-M2. Direct sequencing of the PCR products was performed on all samples and the nucleotide sequences were used to generate partial consensus of 16-23S rRNA, which were analyzed with the BLASTn program. In all the isolates evaluated, the presence of a common band at the height of 620 bp was visualized and the AFLP profile showed that there is homology when comparing the sequences corresponding to the 16-23S rDNA of X. albilineans of the different isolates; the phylogenetic analysis reflected a grouping of the isolates of the species, regardless of its geographical origin, different from others of the genus Xanthomonas. The comparison of the Cuban isolates with eight strains published in GenBank, showed marked similarity with them, with 100% nucleotide identity and percentage of coverage with a genome fragment of the GPE PC73 strain from Guadalupe, KO905 from the United States and a strain from Brazil. The predominance of the generalized circulation in Cuba of a single serovar is presumed.},
 year = {2021}
}

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TY  - JOUR
T1  - Xanthomonas albilineans (Ashby) Dowson: Study of Its Genetic Variability in Sugarcane Samples in Cuba
AU  - Yosel Perez Perez
AU  - Jose Ramon Perez Milian
AU  - Maria La O Hechevarria
AU  - Yaquelin Puchades Izaguirre
AU  - Yenima Pellon Guzman
Y1  - 2021/06/25
PY  - 2021
N1  - https://doi.org/10.11648/j.be.20210501.15
DO  - 10.11648/j.be.20210501.15
T2  - Bioprocess Engineering
JF  - Bioprocess Engineering
JO  - Bioprocess Engineering
SP  - 23
EP  - 27
PB  - Science Publishing Group
SN  - 2578-8701
UR  - https://doi.org/10.11648/j.be.20210501.15
AB  - Four isolates of the phytopathogenic bacterium Xanthomonas albilineans (Xa) from samples taken in commercial fields of different Cuban provinces and two from the cultivar L55-5, artificially infected in the Camagüey and Matanzas Experimental Stations, were used for the extraction of total nucleic acids according to the CTAB protocol. They were amplified by PCR using four primer combinations: A: P1-M1, B: P1-M2, C: P2-M1 and D: P2-M2. Direct sequencing of the PCR products was performed on all samples and the nucleotide sequences were used to generate partial consensus of 16-23S rRNA, which were analyzed with the BLASTn program. In all the isolates evaluated, the presence of a common band at the height of 620 bp was visualized and the AFLP profile showed that there is homology when comparing the sequences corresponding to the 16-23S rDNA of X. albilineans of the different isolates; the phylogenetic analysis reflected a grouping of the isolates of the species, regardless of its geographical origin, different from others of the genus Xanthomonas. The comparison of the Cuban isolates with eight strains published in GenBank, showed marked similarity with them, with 100% nucleotide identity and percentage of coverage with a genome fragment of the GPE PC73 strain from Guadalupe, KO905 from the United States and a strain from Brazil. The predominance of the generalized circulation in Cuba of a single serovar is presumed.
VL  - 5
IS  - 1
ER  -

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